Do you create or customize products?

What is a single-stranded DNA ladder?

What is the advantage of a single-stranded DNA ladder over the conventional ladder?

Yes. Simplex Sciences is proud of our flexibility for innovation. If you would like us to make a product or customize an existing product, please send us an email. We will decide whether it falls within our capacities and whether we can scale the product. If we decide to create the product, we will reply back and give you an estimate on the shipping date.

Future products are in the works, including an ss500 ladder. If you are interested in this product, we encourage you to subscribe to Simplex Sciences' newsletter in the 'Contact Us' section.

At the most fundamental level, our single-stranded DNA ladders can be used as size markers for single-stranded DNAs or RNAs. Through extensive customer analysis and outreach, single-stranded DNA ladders have clear applications to researchers working in the fields of synthetic biology, DNA/RNA biology, DNA sequencing, DNA nanotechnology, among other Essentially, DNA has the potential to cut itself through a process called self-hydrolysis. This recent discovery by Yale professor Ronald Breaker allows DNA to be manipulated in unique ways. Through proprietary protocols, we can harness DNA’s self-hydrolysis capability to control where and when DNA cuts itself. This acts as a shortcut in the production of many possible products, including our primary product line: the single-stranded DNA ladders.

There are two clear benefits. First, dsDNA ladders are not particularly accurate size controls for single-stranded DNA ladders, with regards to visualizing or determining the size of viral ssDNA genomes, donor oligos for CRISPR (and other gene editing techniques), or ssDNA constructs for nanotechnology applications. Thus, you need a single-stranded DNA ladder as a size control. The key difference is that ssDNA and dsDNA have different electrophoretic mobility characteristics, so an appropriate size control of the same type of DNA is necessary. Second, in terms of restaining, we recommend SYBR Gold, as it is a stain specifically developed for ssDNA visualization. By contrast, ethidium bromide (EtBr) intercalates between the strands of dsDNA, so it doesn’t work well for visualizing ssDNA or our single-stranded DNA ladders.